Answer:
The results from this experiment conclude that all of the resulting bacteria contain the pUC18 plasmid without the human gene.
The method of genetic engineering might seem very easy but in actual it is a very difficult task and most of the time a person won;t get the expected results. The chances of a vector to actually take up a recombinant DNA are very less. Even after this, the chances of a recombinant plasmid to enter a bacterial cell by transformation are very few.
The "scissors" of the molecular biology are: DNA Restriction enzymes.
A restriction enzyme is a protein capable of cleaving a DNA fragment at a characteristic nucleotide sequence called a restriction site. Each restriction enzyme thus recognizes a specific site. Several hundred restriction enzymes are currently known.
Naturally present in a large number of species of bacteria, these enzymes have become important tools in genetic engineering.
The "glue" of the molecular biology are: DNA ligase
In molecular biology, DNA ligases are ligase-class enzymes that catalyze the formation of a phosphodiester bond between two segments of DNA. DNA ligases are involved in several essential cellular processes of DNA metabolism: in DNA replication, suture of Okazaki fragments, and in DNA repair and homologous recombination.
The use of these tools in molecular biology: Cloning
Molecular cloning is one of the bases of genetic engineering. It consists of inserting a DNA fragment (called insert) in an appropriate vector such as a plasmid for example. The new plasmid thus created will then be introduced into a host cell, generally the Escherichia coli bacterium. This will then be selected and multiplied to obtain a large amount of the plasmid of interest. Cloning a gene involves inserting it into a plasmid. A clone will be the bacterial transformant that contains this particular plasmid. In this case we speak of clone because all the individuals of the bacterial colony are genetically identical. Molecular cloning is thus different from reproductive cloning (creating an individual genetically identical to another but of a different age) or therapeutic cloning (making tissues from stem cells to perform transplants compatible with the recipient).
Molecular cloning requires restriction enzymes capable of cleaving the DNA, and DNA ligase capable of re-gluing the DNA fragments. Ligase was isolated for the first time from T4 bacteriophage. This enzyme is involved in the repair and replication of DNA. It can bind DNA fragments with compatible sticky ends. At higher concentration, this enzyme is also able to bind two ends of DNA as shown here. T4 DNA ligase works using ATP and Mg ++. It has an activity optimum of 16 ° C, but remains active at room temperature.
Answer:
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Explanation:
Replication is an essential process because, whenever a cell divides, the two new daughter cells must contain the same genetic information, or DNA, as the parent cell. ... Once the DNA in a cell is replicated, the cell can divide into two cells, each of which has an identical copy of the original DNA.
Essential services, such as water purification, nutrient cycling, and plant pollination are all examples of earth's Ecosystem services.
The ecosystem is defined as the interaction between the biological communities with its physical environment. The services provided by the ecosystem are termed as ecosystem services. The ecosystem with the help of its different components performs water filtration, recycling of the nutrients, pollination, seed dispersal etc. These all services provided by the ecosystem comes under the category of ecosystem services.
It is neutrons for sure have a great day
