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It is from adult bone marrow.
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There are 27 x 10⁷ cells in total for the given conditions of cell culture.
Since it is given that the cell culture contains 3 x 10⁵ cells per ml and there are 12 culture flasks in total. It is also given that each of the culture flask holds 75 ml of culture.
Therefore, to calculate the total capacity of the flasks the total number of flasks should be multiplied with the individual capacity of a flask:-
75 x 12 = 900 ml
Now, in order to calculate the total number of cells in the given culture., the total capacity of the flasks i.e. 900 ml should be multiplied with 3 x 10⁵ cells per ml in the following manner:-
900 x (3 x 10⁵) = 2700 x 10⁵ = 27 x 10⁷ cells
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No, even after several days of incubation, you shouldn't detect any symptoms of microbial growth in a sterile medium.
<h3>What is the microbial growth in the sterile medium?</h3>
The deliberate introduction of germs into a sterile growing medium is known as immunization. When there are no living creatures present, a substance is sterile; undesirable bacteria are said to be contaminated. The use of aseptic procedures helps keep growing media from being contaminated. Reduce the amount of time that cultures and growth media are exposed to the outside world. Clean the work area both before and after each use. Avoid breathing or touching the stock cultures or sterile culture media. Before used, loops, needles, pipes, and other items should be sanitized. The tube caps should be held in your hand while inoculating and not placed on the table while using tubes.
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Are cracks in Earth’s crust cause rocks to break and move are younger that surrounding rocks