Answer:
A protein is a long chain of amino acids folded in to a specific shape
Yₒᵤᵣ ₐₙₛWₑᵣ ᵢₛ .:
The number of floral parts (PETALS) is the defining characteristic of the two types of angiosperms (MONOCOTS & DICOTS).
ᴱˣᴾᴸᴬᴺᴬᵀᴵᴼᴺ .: ☯
The number of petals in monocts are in multiple of three (i.e. 3,6 or 9)
While that in dicots are in miltiple of four or five (i.e. 4,5,8 or 10).
Answer:
Use a step wise process
Explanation:
In order to find the presence of the recombinant protein:
- Culture the cells that you believe harbors the plasmid for the recombination protein (+) and also cells that do not (-). This is your control.
- Extract the plasmid from both strains.
- Observe the plasmid map and the area of your insert. Use this to select the restriction enzymes at the beginning and the end of the YFG gene. You can also use a restriction site inside the gene but not in the plasmid for better control.
- Perform the restriction enzyme digest on both (+) and (-) according to your protocol and separate using gel electrophoresis.
- Observe the separate bands that you see. In the (+) there should be a band that is the size of your insert and a larger band that is the size of your plasmid control band and in the (-) there should be a single band that is the size of the plasmid but non the size of the insert.
Answer: The purification gave evidence of the identity of the transforming material, meaning that the possible transforming substance was narrowed down.
Explanation: Removing lipids and carbohydrates suggest that these are not the transforming materials and removing protein suggests that this is also not a transforming material.
The prediction is based on certain observations and some research that you obtain, which helps you make one of these. Your prediction should be somewhat accurate according to the research and observations, and this is also separate from a hypothesis.
