1answer.
Ask question
Login Signup
Ask question
All categories
  • English
  • Mathematics
  • Social Studies
  • Business
  • History
  • Health
  • Geography
  • Biology
  • Physics
  • Chemistry
  • Computers and Technology
  • Arts
  • World Languages
  • Spanish
  • French
  • German
  • Advanced Placement (AP)
  • SAT
  • Medicine
  • Law
  • Engineering
AlexFokin [52]
3 years ago
14

After applying the selection process, your bacterial cells may contain at least three different types of common possible recombi

nants.
a. What restriction enzyme(s) could you use to distinguish between the various recombinants?
b. Draw and label a rough sketch of an agarose gel pattern that you would see if you performed the restriction digests of possible AmpR recombinants. Also provide plasmid maps to show how you calculated the sizes of fragments. Be sure to draw a DNA ladder and label fragments with their estimated size.

Biology
1 answer:
Tju [1.3M]3 years ago
6 0

Answer:

1. Restriction enzymes are endonucleases, that is, enzymes that digest nucleic acids. Restriction enzymes recognize specific sequences of nucleotides in a DNA strand. Their use allows the detection of point mutations in DNA and eliminates the need for subcloning and sequencing.

Traditionally, four types of restriction enzymes are recognized, designated I, II, III, and IV, which differ primarily in structure, cleavage site, specificity, and cofactors.

b. answer is attached.

c. Multiplying the genome size by the frequency equals the number of restriction fragments produced, or (2.5 x 107 bp)(2.56 x 10-4 bp-1) = 6400 fragments. Divide the genome size by the number of fragments to determine the average fragment size, or 2.5 x 107 bp/6400 = 3.9 x 103 bp.]

You might be interested in
HELLPOOOOP PLZZZ 101010101010010
Leviafan [203]

Answer:

everything but bacteria

Explain:

Bacteria is a type of cell as the rest are made of cells

5 0
3 years ago
Read 2 more answers
Can plants survive in space?
lana [24]
No they can't there's no air in space space is like a vacuum it suck up all the air
4 0
3 years ago
Read 2 more answers
In sexual reproduction, two different strains of hyphae called _____ and _____ strains fuse nuclei and produce a zygote.
Margarita [4]
C. is the answer your looking for in that order.
3 0
3 years ago
Read 2 more answers
Which common practice puts the nurse at liability for invasion of patient privacy?
harina [27]

Answer;

-The nurse gives information about the patient to telephone callers who inquire about the patient.

Explanation;

-Medical care requires the invasion of privacy. Patients must expose their innermost thoughts, their bodies, and their sickrooms to strangers. But to protect human dignity, health providers should limit invasions to those necessary to accomplish the goals of their patients.

-Invasion of privacy and confidentiality are of critical legal and medical concern. The right to privacy means that a client has the right to expect that his or her property will be left alone. Healthcare individuals may be charged with trespassing, illegal search and seizure, or releasing private information (even if the information is true).

8 0
3 years ago
PLEASE PLEASE HELP (20 points)<br> I will mark Brainliest
Nitella [24]

Answer:

Hope it helps :)

7 0
3 years ago
Other questions:
  • Describe the parts the accessory organ play in the digestion of food
    7·1 answer
  • Any organism in which the dna has been altered using recombinant dna technology is considered a genetically modified organism. a
    9·1 answer
  • For a 24-year-old male patient who received immediate care in the hospital emergency room, the coder should refer to the e/m cat
    5·1 answer
  • The science of the classification of organisms is _____. a taxon a taxonmist a species taxonomy
    8·2 answers
  • The human population is increasing because of:
    6·1 answer
  • Why doesn t a skin cell make crystallin protein mastering biology?
    11·1 answer
  • What is non living or not produced by living things
    5·1 answer
  • A body part that must resist unpredictable stress from multiple directions would likely be composed of which tissue
    7·1 answer
  • If cells were fed glucose with radioactive 14c, what other molecule besides co2 would you expect to be radiolabeled? see section
    9·1 answer
  • Margo is designing an experiment to investigate the rock cycle. She has written the following steps.
    11·1 answer
Add answer
Login
Not registered? Fast signup
Signup
Login Signup
Ask question!