Answer:
Denaturation process: The DNA template
Annealing process: Primers
Elongation process: dNTPs and Taq polymerase
Explanation:
For the denaturing process, the only ingredient that is required is the DNA template that will be separated from a double helix (or double strand) into a single strand, by increasing the temperature to 95 C, (at this temperature the hydrogen bonds that keep together the double stranded break). After the double strand is denatured, the following process is annealing. For this, the required ingredient are the primers; these primers will hybridize or anneal according to the nucleotide complementarity to the single strand of the DNA. Finally, for the Elongation process, you will require the Taq polymerase and the dNTPs. The enzyme will synthesize or “generate” a new strand of DNA based on the DNA template, using the provided dNTPs in the direction 5’ to 3’.
I hope this clarify you inquiry.
The reaction of a particular endothermic reaction whether in a negative or positive entropy change is always spontaneous. Temperature differences will be spontaneous throughout the reaction but one thing is constant, heat will always be produced within the said reaction.
Using the telescope, Galileo discovered the mountains on the moon, the spots on the sun, and four moons of Jupiter. His discoveries provided the evidence to support the theory that the earth and other planets revolved around the sun.
