Answer:
The concentration, in cells/ml, of the original sample is 52500 cells/ml
Explanation:
Given
- Average number of cells in 1/25 mm^2 sized box (considering 1 box) is 7
- The depth of each box of a hemocytometer is 0.1 mm
To find the Volume:
Volume = 1/25 mm^2 * 0.1 mm^3 = 0.004 mm^3
Concentration of cells in this volume
[Cells in the estimated volume] = 7/0.004 mm^3 = 1750 cells/mm^3.
Knowing 1 mm^3 = 1 ml,
cell count = 1750 cells/ml.
Using the dilution factor 1:30 to correct the calculation
Final cell count after correction = 1750* 30 = 52500 cells/ml
