Describe the principle of MPN for enumerating bacteria from a natural sample.

Biology

1 answer:

KengaRu [80]3 years ago

5 0

Answer:

MNP stands for the most probable number which is used for finding out the concentration of viable bacteria in a sample. It is commonly used to check the quality of water and its safety. Usually, the presence of fecal coliform bacteria is checked in this test.

Principle of MNP: The sample which is to be tested is diluted serially and then inoculated into a broth medium containing lactose. If coliforms are present in the tube than it will consume lactose and release acid and gas.

Color change represents acid production and gas trapped in the Durham tube represents the gas production by bacteria. Then the number of tubes at each dilution that showed positive results are compared with standards to determine the most probable number of the bacteria.

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According to this question, a gene coding for the number of fingers in humans is involved. The allele for six fingers (F) is the dominant trait while the allele for five fingers (f) is the recessive trait.

If a cross between a heterozygous father that posseses a genotype of Ff and a homzygous mother that posseses a genotype of ff, the following offsprings will be produced:

This shows that the probability of the offspring of a heterozygous father and homzygous mother having five fingers is ½ (50%).

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