Answer/Explanation:
(1) a mutation in the coding region, resulting in an inactive protein
To check to see if there is a mutation, you could extract the DNA from the cancer cells and then perform PCR to amplify the gene of interest. You could then perform sanger sequencing and compare the sequence to the normal gene to see if a mutation is present. To test the effect of the mutation, you would want to see if an active protein has been formed.
To see if a normal sized protein has been formed, you could perform a western blot, comparing the protein band to the WT protein band. If the protein is absent or much smaller, it is likely not a functional protein.
(2) epigenetic silencing at the promoter of the gene, resulting in reduced transcription.
To check for changes in the epigenetic landscape of the promoter, you could perform chromatin immunoprecipitation by extracting the chromatin from the tumour cells and using antibodies for different chromatin marks to see what has changed between the normal cells and the tumor cells. E.g. H3K9me3, H3K27me3. You would perform a pull down with the antibody of interest and then PCR for your promoter to specifically look at changes at that gene compared to normal cells. To test DNA methylation, you could perform bisulfite sequencing.
To see how transcription is affected, you could extract RNA from the tumor and normal cells, and compare the levels of RNA between the two samples by qRT-PCR
Answer:
Explanation:
Species: a group of living organisms consisting of similar individuals capable of exchanging genes or interbreeding.
Population: a particular group or type of people or animals living in a place.
Gene pool: the stock of different genes in an interbreeding population.
Mutations: the changing of the structure of a gene, resulting in a variant form that may be transmitted to subsequent generations, caused by the alteration of single base units in DNA, or the deletion, insertion, or rearrangement of larger sections of genes or chromosomes.
Lateral Gene Transfer: the movement of genetic material between unicellular and/or multicellular organisms other than by the transmission of DNA from parent to offspring (reproduction).
Single-gene Traits: when a trait is linked to one gene-pair that consists of two alleles.
Polygenic Traits: is one whose phenotype is influenced by more than one gene.
It is an advantage because they approach their environments from all sides equally.
Answer:
Properties of Life. All living organisms share several key characteristics or functions: order, sensitivity or response to the environment, reproduction, growth and development, regulation, homeostasis, and energy processing. When viewed together, these characteristics serve to define life.
Very glad I could help!!
Answer:
Gravity is the main force responsible for mass movements. Gravity is a force that acts everywhere on the Earth's surface, pulling everything in a direction toward the center of the Earth. On a flat surface, parallel to the Earth's surface, the force of gravity acts downward
Explanation:
