Answer:
1) M1 is heterozygous since it contains 2 bands, which means that it contains one recessive and one dominant allele
P1 is homozygous for this locus, since it contains a single band or is known to be homozygous for the locus or gene.
P2 is hetozygous for this locus because two bands appear in which each band represents one allele for the gene.
ChildC contains two gel bands, representing two alleles and is therefore heterozygous
P1 is the father of the child C1, if we look at the gel, each father shows a similar band, one coinciding with the mother and the second with the father p1
As for the paternity study, the PCR test is not useful, since it provides asymmetric results, thus only one strand information will be provided, and because the DNA is double-stranded, a more complete study is required.:
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Answer:
The pUC18 refers to a polylinker that is featured by enhanced multiple restriction locations. The enhanced number of restriction sites would upsurge the chance of the target DNA sequence being presented into the plasmid. If the sequence of the DNA is administered efficiently and obstructs the sequence of the LacZ gene in the restriction site, the bacteria will not be able to generate B galactosidase that cleaves X-gal.
When the X-gal in the medium does not get cleaved, the colonies become white. In case, if the DNA was not effectively presented into the host DNA and the LacZ gene can generate B galactosidase, X-gal will get cleaved and the colonies will be blue.
As the white colonies will be the hosts comprising the recombinant DNA, and pUC18 enhances the chance of DNA getting inserted, the white colonies will most probably comprise the recombinant pUC18.
Explanation:
To ensure ratification of the document, the Federalists offered concessions, and the First Congress proposed a Bill of Rights as protection for those fearful of a strong national government. The Bill of Rights came into effect in December 1791, after ratification by three-fourths of the state legislatures.