Based on the given information, 0.1 ml is the amount of bacterial culture plated, 10^-5 is the dilution factor and the number of bacterial colonies produced is 223.

A) 223 is the number of colonies produced when 0.1 ml of the culture is plated. Therefore, the number of colonies produced when 1 milliliter of bacterial culture plated us (223/0.1)*1 = 2230

The calculation of the CFU/ml is done by using the formula,

CFU/ml = Number of colonies per ml plated / dilution factor

Thus, 2230/10^-5

= 2230 * 10^5 or 2.23 * 10^8 CFU/ml

B) The number of colonies, which would grow on a plate, which is inoculated with 0.1 ml volume of 10^-7 dilution from the similar bacterial stock will be calculated as,

CFU/ml = Number of colonies per ml plated/ dilution * volume plated.

2.23 * 10^8 CFU/ml = Number of colonies per ml plated / 10^-7 * 0.1

Number of colonies per ml plated = 2.23 * 10^8 * 0.1 / 10^7 = 2.23 or 2 colonies.

8 0

3 years ago

Of energy-absobing reaction and energy-releasing reaction which one is more likely to start spontaneosly and why?

zmey [24]

Answer:

Energy releasing reaction = exergonic reaction (negative G) is more likely to be spontaneous.

Explanation:

Exergonic reactions do not need any input of energy, and therefore are happening spontaneously. On the other hand, endergonic reactions need an energy input. These reactions are often paired = coupled reactions.

Hope this will help :)