Answer:
long are W which means that they are stronger than the w trait of short.
1)the big word means that it will have two W traits, (or W,W), and heterozygous will mean that the seal will have one W and one w, (or W, w).
your Punnett Square will look like this W W which means that 100% of
these W W W W W
seals w W w W w
will have long.
2)Heterozygous and a short will look like this
w W w w w
w w w w w
which means that only 25% will have long and 75% will have short, this is due to how W traits will almost always beat recessive traits.
3)Now one is, (H) and two is, (h). Two Hybrid means that they are Heterozygous. This will make your Punnett Square look like this
H h
H H H H h
h H h h h
the possible Genotypes willbe H H, H h, h h.
Phenotypes, looktraits willbe One,and Two.
Answer:
Given:
Number of colonies on plate = 40
Amount of DNA used for transformation = 100ng
Volume of competent cells used = 100ul
Total volume = 1ml (1000ul)
Volume plated for growth = 200ul
The amount of cells transformed per 1 µg of DNA is called the transformation efficiency
(CFU is colony forming units) => (number of colonies on plate/ng of DNA plated) X 1000 ng/µg = CFU/µg of DNA
Amount of DNA used for plating = 100ng * (200/1000)
= 100ng * (1/5)
= 100/5 = 20ng
Efficiency calculation = 40 colonies / 20ng = 2 colonies/ng
(2 colonies/ng) * (1000 ng/µg) = 2000 CFU/ug DNA
Transformation efficiency = 2000 Transformants / ug DNA
= 2 x 10³ Transformants / ug DNA
Explanation:
Efficiency of transformation is highest in the 100 pg-1 ng range, so Low transformation efficiency could be because of high amount of DNA used for transformation.
It’s true. I don’t know what else to add
Blank 1 = iron..blank 2= red meat
