Yes taking a time out is an effective anger management tool
It increased slightly if you measure precisely but if you round it did not
Answer:
RNA polymerase binds to the promoter
Polymerase initiates RNA synthesis at the start point on the template strand
RNA polymerase moves downstream unwinding the DNA
RNA transcript is released and polymerase detaches from the DNA
The pre-RNA undergoes processing
Explanation:
The promoter is the specific DNA sequence that serves as a binding site for RNA polymerase. The enzyme RNA polymerase recognizes the promoter sequence and binds to it to begin the process of RNA synthesis. The process of RNA synthesis begins at the start site where the process of the unwinding of DNA duplex is initiated.
The unwinding separates the two DNA strands at the start site where RNA polymerase begins the process of synthesis of the new RNA strand. The newly formed RNA strand and RNA polymerase are released from the template strand after the termination of the process.
In eukaryotes, the primary RNA transcript undergoes the process of splicing of introns, the addition of poly-A tails and 5' cap which in turn transform it into the mature mRNA.
Hello. This question is incomplete. The full question is:
"You determine that you have only 3 copies left of an important DNA fragment, so you decide to amplify it. Using flanking primers, how many PCR cycles would you have to run to generate over one billion (10^9) copies of the fragment?
"
Answer:
Approximately 29 cycles of PCR would be required.
Explanation:
As you may already know, PCR is a technique used in molecular biology that allows part of a DNA molecule to be recycled into millions of copies.
PCR allows this replication to be done through cycles. Each PCR cycle lasts about 2 minutes and allows the DNA molecule to undergo the separation of the strands, the binding of the primers and the synthesis of new DNA strands through DNA polymerase. This cycle is usually repeated 32 times, but this number may change depending on the number of copies the researcher thinks is necessary.
In this case, if a researcher wants 10 ^ 9 copies of DNA, he must do the following calculation to find the number of PCR cycles needed:
32 ^ x = 10 ^ 9
x = 28.3 = approximately 29 cycles.
