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love history [14]
3 years ago
6

The technique of Sanger sequencing takes advantage of the fact that dideoxynucleotides (nucleotides in which the 3′ hydroxyl gro

up is absent) act as:
a) palindromic sites.
b) restriction sites.
c) chain terminators.
d) probes.
e) None of the other answer options is correct.
Biology
1 answer:
Sauron [17]3 years ago
4 0

Ans.

The Sanger sequencing or chain-termination method for sequencing involves use of ddNTPs (dideoxyribonucleotide triphosphates) to identify the sequence of nucleotides in a DNA segment. The ddNTPs are nucleotide derivatives that lack both the 2'- and 3'-hydroxyl group, hence called dideoxyribonucleotides. When a ddNTP is added to a growing chain of DNA, absence of 3'-hydroxyl group leads to termination of growing chain as this ddNTP cannot make phosphodiester bond with a NTP and DNA sequence can be identified.

Thus, the correct answer is option C).

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Prokaryotic organisms are single-celled organisms that lack a cell nucleus, and their DNA therefore floats freely in the cell cytoplasm. To synthesize a protein, the processes of transcription and translation occur almost simultaneously. When the resulting protein is no longer needed, transcription stops. As a result, the primary method to control what type of protein and how much of each protein is expressed in a prokaryotic cell is the regulation of DNA transcription. All of the subsequent steps occur automatically. When more protein is required, more transcription occurs. Therefore, in prokaryotic cells, the control of gene expression is mostly at the transcriptional level.

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