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love history [14]
3 years ago
6

The technique of Sanger sequencing takes advantage of the fact that dideoxynucleotides (nucleotides in which the 3′ hydroxyl gro

up is absent) act as:
a) palindromic sites.
b) restriction sites.
c) chain terminators.
d) probes.
e) None of the other answer options is correct.
Biology
1 answer:
Sauron [17]3 years ago
4 0

Ans.

The Sanger sequencing or chain-termination method for sequencing involves use of ddNTPs (dideoxyribonucleotide triphosphates) to identify the sequence of nucleotides in a DNA segment. The ddNTPs are nucleotide derivatives that lack both the 2'- and 3'-hydroxyl group, hence called dideoxyribonucleotides. When a ddNTP is added to a growing chain of DNA, absence of 3'-hydroxyl group leads to termination of growing chain as this ddNTP cannot make phosphodiester bond with a NTP and DNA sequence can be identified.

Thus, the correct answer is option C).

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8. In chickens, brown feathers are dominant over red feathers. Suppose we cross two heterozygous chickens.
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Answer:

Crossing two chickens heterozygous for the trait feather color, when brown feathers are dominant over red feathers, will produce offspring with brown and red feathers in a brown:red phenotypic ratio of 3:1.

Explanation:

Knowing that in chickens brown feathers are the expression of the dominant allele (B) and the recessive allele is red (b), crossing two heterozygous individuals (Bb) will result in offspring that are likely to have brown or red feathers.

The cross can be represented by a Punnett Square:

<u>Bb X Bb cross </u>

alleles     B    b

B             BB Bb  

b             Bb bb

Where the result is:

<u>Genotypes</u>:  

  • Bb, 50%
  • BB, 25%
  • bb, 25%

<u>Phenotypes</u>:

  • Brown feathers, 75%
  • Red feathers, 25%.

In this case, the probability of having brown or red feathers is 3:1.

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