The correct answer is option (C) cytoplasm, interstitial fluid.
All cells are bound by the semi-permeable plasma membrane made up of the lipids and proteins. Cytoplasm is the material within the living cells excluding the nucleus. It consists of the cytosol and the cell organelles. Interstitial fluid refers to the fluid between the cells. It is the extracellular fluid that fills the spaces between the cells. The presence of the plasma membrane acts as a barrier and separates the cytoplasm of the cells from the interstitial fluid around the cell.
The DNA is elongated in the 5'-to-3' end direction, meaning that the DNA polymerase adds a new nucleotide to the free 3' end OH- group. The DNA polymerase will catalyze formation of the bond between the 3' OH- group at the end with the 5' phosphate group of the new nucleotide forming the phosphodiester bond.
