You have a dish of mammalian cells in culture that are all about to enter mitosis (it is a synchronously replicating culture of
cells). You add to the dish a drug that is a potent inhibitor of RhoA activity. Two hours later you analyze your cells under the microscope. Which of the following best describes what you might observe? a. All cells appear to be in telophase. b. All cells appear to be at metaphase. c. Around 50% of cells appear to be in anaphase A. d. All cells have completed mitosis e. Around 50 % of the cells have undergone apoptosis. .
RhoA is a protein which belongs to the Rho GTPase family proteins and related Ras superfamily. The Rho A protein is involved in the cell division before the cytokinesis as they are involved in the formation of cytokinesis structures like the actin-myosin rings and the contractile ring formation.
In the given question, if the drug inhibits the activity of RhoA, therefore, cytokinesis will be affected and the cell will complete all the stages of Mitosis except cytokinesis. The cells will be mostly observed in the telophase as cytokinesis will take place after that.
