1st container: isotonic solution, the concentrations of solutes that cannot cross the plasma membrane are the same on both sides of the membrane in this solution, * its equal
middle container: hypotonic, a solution that has a LOWER concentration of solutes than the cytosol inside the RBC's - water molecules enter the cells faster than they leave causing the RBC to swell and eventually burst *hemolysis
last container: hypertonic solution, has a HIGHER concentration of solutes than does the cytosol inside RBCs, water molecules move out of the cells faster than they enter causing the cells to shrink *crenation
Cation-exchange chromatography is used when the molecule of interest is positively charged on the neutral pH. The stationary phase is than negatively charged and positively charged molecules are loaded to be attracted to it. So, positively charged peptides will elute the last.