The introns of protein-encoding nuclear genes of higher eukaryotes almost invariably begin (5') with GT and end (3') with AG. In addition, the 3'subterminal A in the “TACTAAC box” is completely conserved; this A is involved in bond formation during intron excision.
Gene therapy is a medical field which focuses on the utilization of the therapeutic delivery of nucleic acids into a patient's cells as a drug to treat disease.
