Answer:
Problems with transfection efficiency or absence of essential mRNA modifications (capping and poly-A tail)
Explanation:
Translation is the process of protein synthesis from RNA in which the genetic information encoded in a messenger RNA (mRNA) molecule is translated into a protein sequence composed of amino acids. Translation has three steps: initiation, elongation, and termination. During translation, different elements are required to initiate, continue and complete the process, which include essential molecules such as amino acids, mRNA, tRNAs, ribosomes, energy-carrying molecules (i.e., ATP, GTP), initiation factors (i.e., eIF1A, eIF3, etc) and elongation factors (eEF-2, EIF5A). For example, humans can't synthesize nine essential amino acids (i.e., threonine, tryptophan, phenylalanine, valine, methionine, leucine, histidine, isoleucine, lysine), thereby it is imperative to include these amino acids to synthesize proteins composed of them. On the other hand, it is also fundamental to take into account that the efficiency of transfection of the molecules required during translation, as well as the absence of modifications in the mature mRNA sequence, may alter the process in vitro.
<span>D.
Demographers acquire all that data in questionnaires and surveys.</span>
Answer:
A) It helps to separate the chromosomes
Explanation:
Answer:
military strategies and cultural assimilation.
Explanation:
Sargon started his career in the Sumerian city-state of Kish as an official who eventually murdered the King of the city-state and seized control of it. Sargon raised an army and with sophisticated military techniques stormed through Mesopotamia to build an empire, became the first emperor of the region. he built the capital city of Akkad and despite their difference, Akkadian adopted Sumerian culture. This cultural assimilation of the conquered people has helped them to adjust better in the large empire.
