Nicked circle is the reason.
Explanation:
During recombinant DNA technology the gene of interest is ligated to plasmid DNA by excision with molecular scissors or endonuclease enzyme and ligated with enzyme ligase.
Enzyme ligase catalysed by ATP seals the nicks by making phosphodiester bonds in the ds or ss DNA and release of ADP.
The ligation in the plasmid did not occur properly hence the replication is interrupted hence no new plasmids with gene of interest replicated in the ligation carried out.
Answer:
The options
A) "Medications are needed to prevent heart failure."
B) "I will take aspirin, atenolol, and captopril indefinitely."
C) "My blood pressure should be less than 130/80 mm Hg."
D) "Daily estrogen will prevent another heart attack."
The CORRECT ANSWER IS D)
D) "Daily estrogen will prevent another heart attack."
Explanation:
A reduction in the natural hormone estrogen levels could be a causative factor in heart disease increase within post-menopausal women. Estrogen is thought to show a positive impact on the inner layer of artery wall, functioning in such a way to keep blood vessels elastic.
In spite of the advantages of estrogen, the American Heart Association speaks against employing postmenopausal hormone therapy to decrease the likelihood of coronary heart disease or stroke as some research works have revealed that it vividly do not decrase the risk of heart attack but increases the likelihood of blood clots and stroke.
Answer:
oxygen
Explanation:
oxygen isn't necessarily a characteristic of life. it's more like an aspect of life
In a research, the independent variable is the one which causes a change in the dependent variable. In this case, the nurse visit in hoped to cause reduction of doctor visits. That means the doctor visit is dependent variable and nurse visit will be the independent variable<span>.</span>
<span>In contrast to a cerebral concussion, a cerebral contusion </span>involves physical injury to the brain tissue. Cerebral Concussion is a <span>Traumatic brain injury.</span>
