Answer:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder
Load 12 ul on the gel.
Explanation:
DNA Ladder concentration = 1000 ug/ml
1000 ug DNA in 1 ml DNA Ladder solution → 150 ng DNA = 0.15 ug DNA in..... 0.00015 ml = 0.15 ul DNA Ladder solution
6x DNA Loading Buffer → it has to be diluted by an equal volume 6 times (1 ul LB + 1 ul distilled H2O)
An appropriate volume to load on an average agarose gel is 12 ul, so:
2 ul Loading Buffer + 9.85 ul dH2O + 0.15 ul DNA Ladder = 12 ul
But since 0.15 ul is a very small volume and mistakes could be made while measuring it, let's make double:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder = 24 ul
And load half of that solution (12 ul) on the gel.
Answer:
increases levels of greenhouse gases
Explanation:
Rainfall can cause erosion both when the rain hits the surface of the Earth, called splash erosion, and when raindrops accumulate and flow like small streams. Rivers can create a significant amount of erosion over time. They break up particles along the river bottom and carry them downstream. One example of river erosion is the Grand Canyon which was formed by the Colorado River. Ocean waves can cause the coastline to erode. The shear energy and force of the waves causes pieces of rock and coastline to break off changing the coastline over time. Large floods can cause erosion to happen very quickly acting like powerful rivers.