Answer:
a. the virus must be able to cross-link red blood cells directly.
Explanation:
Viral hemagglutination assay is a method which is used for the quantification of the relative concentration of the viruses.
The principle on which the hemagglutination assay works is as follows:
- There are sialic acid receptors present on the surface of the red blood cells which will bind to the haemagglutinin glycoprotein present on the surface of the viral particle.
- Due to the binding of the sialic acid receptors with haemagglutinin particle there will be cross-linking of the red blood cells by the viral particles and a lattice formation will take place.
- The lattice will remain suspended in the solution however if the concentration of the virus particles will be less, the lattice will settle down in the well.
Thus, this method allows knowing the relative concentration of the virus.
<em>Due to its basic principle of cross-linking this method works well for only a few viruses such as influenza virus. </em>
Hi
Below are five major steps of DNA or gene cloning:
1: You chose the gene or specific piece of DNA you need to clone and cut the gene with restriction enzymes from the source organism.
2: You need to choose a vector for the process of cloning and you will cut the vector with same restriction enzymes through which you have cut your target DNA sequence to be inserted into the vector.
3: You place the target gene into the vector and join or seal the gene with vector by using an enzyme called DNA ligase.
4: You introduce the vector with your target gene into a suitable host organism such as yeast or bacteria through the process of Transformation. In this process host organism takes up the vector containing your target gene and starts replicating the target DNA along with their own DNA and thus creating millions of copies of target gene .
5: In the last step, the DNA or target gene is isolated from host organism and purified and is ready to be used since its quantity has been enormously increased through the process of cloning.
The cloning is also called as recombinant DNA technology and is the main process that is being used in the production of insulin for diabetes patients. You can see below image for better understanding.
Hope it help!
Answer: angular distance north or south from the celestial equator measured along a great circle passing through the celestial poles
Explanation:
The 2% of the insects will begin to reproduce and grow their population exponentially and in time enable the population to recover. This is if the population is large enough to enable random mating. The 98% reduction in population drastically reduces competition for the remaining individuals hence giving them abundant resources for them to thrive.
