Question

A student needed to prepare 500mL of 1X TAE buffer to run a QC gel. The stock solution in the lab is 5X TAE. What volumes of stock TAE and water are needed to prepare the 1X working TAE buffer?

Answer 1

Answer:

you need 100ml of 5X TAE and 400ml of water.

Step-by-step explanation:

You need to use a rule of three:

$C_1V_1=C_2V_2$

where:

$\left \{ {{C_1= 5X} \atop {C_2=1X}} \right.$

and

$\left \{ {{V_1 = V_{TAE}} \atop {V_2=500ml}} \right.$

Therefore:

$V_{TAE} = \frac{V_2*C_2}{C_1}$

$V_{TAE} = 100ml$

Then just rest the TAE volume to the final Volume and you get the amount of water that you need to reduce the concentration.

Answer 2

Answer:

You need 750 ml deionized water and 57.1 ml glacial acid and 100 ml of 0.5 M  EDTA

Step-by-step explanation:

The stock solution is made by making a concentrated solution of TAE by weighing out 242 grams of Tris base. This is then dissolved in approximately 750 milliliters of deionized water. After that, 57.1 ml of glacial acid are added to the 100 l of 0.5 M EDTA. After that, the final volume is adjusted to 1 liter. This will be the stock solution of the buffer solution.