<span>The plasma membrane is both a barrier and gateway between the cytoplasm and ECF. It is selectively permeable—it allows some things through, such as nutrients and wastes, but usually prevents other things, such as proteins and phosphates, from entering or leaving the cell.</span><span>The methods of moving substances through the membrane can be classified in two overlapping ways: as passive or active mechanisms and as carrier-mediated or not. Passive mechanisms require no energy (ATP) expenditure by the cell. In most cases, the random molecular motion of the particles themselves provides the necessary energy. Passive mechanisms include filtration, diffusion, and osmosis. Active mechanisms, however, consume ATP. These include active transport and vesicular transport. Carrier-mediated mechanisms use a membrane protein to transport substances from one side of the membrane to the other. We will first consider the mechanisms that are not carrier-mediated (filtration, simple diffusion, and osmosis) and then the carrier-mediated mechanisms (facilitated diffusion and active transport).</span>
