I think the answer is ozone i hope i am right and hope this helps
Answer:
Random mutations led to evolution of pesticide resistance genes in bed bugs.
Explanation:
Random mutations in genome of bed bug imparted them the pesticide resistance. Since the bed bugs having the mutation of pesticide resistance were able to survive under presence of pesticides, this variation was favored by natural selection. The bugs with pesticide resistance transmitted this trait to their progeny. In time, the bed bug population consisted of most of the bugs having the pesticide resistance.
The evidence would be all the different types of species
The function of tRNA is to read the messages of the nucleic acids, and then translate it into amino acids.