Answer:
Advances in DNA technology have allowed for treatments such as gene therapy in the medical field. Nowadays, there are tools like the CRIPSR-Cas9 - harnessed from bacteria's immune system- that can nip a ‘bad’ region of DNA, which is responsible for disease, and replace it with a proper functioning bit. This is how genetic disorders are going to be addressed in the near future in mainstream medical facilities.
Recombinant DNA technology is being used to create clones of transgenic organisms that produce products of significance in the medical fields. An example is incorporating the insulin gene in bacteria genome. These clones of modified bacteria colonies secrete insulin protein that is purified and injected in diabetic persons to lower their sugar level to normal.
The organization that issued the cred for communication ethics would be the national ethics association. Hope this helps!
Answer:
Given:
Number of colonies on plate = 40
Amount of DNA used for transformation = 100ng
Volume of competent cells used = 100ul
Total volume = 1ml (1000ul)
Volume plated for growth = 200ul
The amount of cells transformed per 1 µg of DNA is called the transformation efficiency
(CFU is colony forming units) => (number of colonies on plate/ng of DNA plated) X 1000 ng/µg = CFU/µg of DNA
Amount of DNA used for plating = 100ng * (200/1000)
= 100ng * (1/5)
= 100/5 = 20ng
Efficiency calculation = 40 colonies / 20ng = 2 colonies/ng
(2 colonies/ng) * (1000 ng/µg) = 2000 CFU/ug DNA
Transformation efficiency = 2000 Transformants / ug DNA
= 2 x 10³ Transformants / ug DNA
Explanation:
Efficiency of transformation is highest in the 100 pg-1 ng range, so Low transformation efficiency could be because of high amount of DNA used for transformation.
Answer:
Anchoveta feed on large zooplankton whereas sardines feed mainly on phytoplankton. Use this information to suggest why sardine numbers are less
likely to collapse during an El Niño event?
Reason why sardine are less likely to collapse during an El Nino event is as a result of sardine survival is neither dependent on zooplankton nor anchoveta rather sardine is dependent on phytoplankton which is not scarce
Explanation: