Why did the heat treated enzyme behave differently than the non heated enzyme
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Answer:
1) After adding 15.0 mL of the HCl solution, the mixture is before the equivalence point on the titration curve.
2) The pH of the solution after adding HCl is 12.6
Explanation:
10.0 mL of 0.25 M NaOH(aq) react with 15.0 mL of 0.10 M HCl(aq). Let's calculate the moles of each reactant.
There is an excess of NaOH so the mixture is before the equivalence point. When HCl completely reacts, we can calculate the moles in excess of NaOH.
NaOH + HCl ⇒ NaCl + H₂O
Initial 2.5 × 10⁻³ 1.5 × 10⁻³ 0 0
Reaction -1.5 × 10⁻³ -1.5 × 10⁻³ 1.5 × 10⁻³ 1.5 × 10⁻³
Final 1.0 × 10⁻³ 0 1.5 × 10⁻³ 1.5 × 10⁻³
The concentration of NaOH is:
NaOH is a strong base so [OH⁻] = [NaOH].
Finally, we can calculate pOH and pH.
pOH = -log [OH⁻] = -log 0.040 = 1.4
pH = 14 - pOH = 14 - 1.4 = 12.6