The largest spider is called goliath bird
Biomes are typically defined<span> by their climate and dominant vegetation, including grassland, tundra, desert, tropical rainforest, and deciduous and coniferous forests.</span>
Answer:
B
Explanation:
Valleys are created by streams of running water
Scientists collect ongoing data from many locations around the world. They then input this data into a mathematical or computer-based model to simulate real-world conditions.
<h3>What is climate forecasting?</h3>
Weather averages and other climatic characteristics are predicted by climate forecasts from a few weeks to a few years in advance. Such forecasts are increasingly being made using thorough models of the Earth's climate system.
Since it aids in forecasting future climate conditions, climatology and weather forecasting are crucial. The likelihood that snow and hail will reach the surface can be calculated using latitude. You may also determine the thermal energy from the sun that a location can get.
Learn more about climate forecasting, here:
brainly.com/question/12516408
#SPJ1
Answer/Explanation:
(1) a mutation in the coding region, resulting in an inactive protein
To check to see if there is a mutation, you could extract the DNA from the cancer cells and then perform PCR to amplify the gene of interest. You could then perform sanger sequencing and compare the sequence to the normal gene to see if a mutation is present. To test the effect of the mutation, you would want to see if an active protein has been formed.
To see if a normal sized protein has been formed, you could perform a western blot, comparing the protein band to the WT protein band. If the protein is absent or much smaller, it is likely not a functional protein.
(2) epigenetic silencing at the promoter of the gene, resulting in reduced transcription.
To check for changes in the epigenetic landscape of the promoter, you could perform chromatin immunoprecipitation by extracting the chromatin from the tumour cells and using antibodies for different chromatin marks to see what has changed between the normal cells and the tumor cells. E.g. H3K9me3, H3K27me3. You would perform a pull down with the antibody of interest and then PCR for your promoter to specifically look at changes at that gene compared to normal cells. To test DNA methylation, you could perform bisulfite sequencing.
To see how transcription is affected, you could extract RNA from the tumor and normal cells, and compare the levels of RNA between the two samples by qRT-PCR
