Definition
A technique which is used to separate, DNA, RNA or protein pieces from each other under the influence of electric field on the basis of their molecular size is known as gel electrophoresis.
Explanation
This method is very reliable for separation of large size molecule (over 1 million Da). Materials which are required for gel electrophoresis include:
1. TAE stock buffer
2. 1% agrose gel
3. Nucleic acid loading dye
4. Ethidium bromide
Procedure:
First prepare a stock solution of TAE buffer by adding appropriate amount of TAE in distilled water. then prepare 1% agrose by adding 1X TAE, some amount of agrose in water and heating it in microwve oven to mix them will. then pour agrose gel on tray and fix comb in it and keep it untill agrose dry. Then remove comb and pour some quantity of nucleic acid along with loading dye and ethidium bromide in each well. EtBr is used for staining nucleic acid.
When sample is poured in all well also pour reference marker in one well for comparison. now connect it with voltage for 30-35 min. After this take gel and see it under UV. a large number of nucleic acid pieces will be seen on gel under UV. those pieces which have small molecular weight will cover more distance compared to those having larger molecular weight.
Answer:
Schizophyta is now commonly known as cyanophyta. Their characteristic are:
They are blue green algae. ...
They have organelle known as the thylakoid which are flattened, which help them in photosynthesis.
They obtain oxygen from the atmosphere for their energy and respiration.
Explanation:
Answer: d. none of the other answers
Explanation:
Enzymes that turn chemical energy into mechanical work are molecular motors.
There are three main components of cytoskeleton including microtubules, actin filaments and Intermediate filaments. Only actin filaments and microtubules carry the motor-driven transport on the cytoskeleton.
The most important feature of intermediate filaments is to provide the plasma membrane with mechanical support where it comes into contact with the extracellular matrix and other cells.
Unlike microtubules and microfilament , intermediate filaments are not involved in motility of the cells, hence do not associat with molecular motors.
Hence, the correct option is d.
The ATP enables the movement of transport proteins to move the ions across a cell membrane by its getting fueled by its hydrolysis
<u>Explanation:</u>
The plasma membrane is a semi permeable membrane that is the movement of molecules across the membrane is constricted. There are 2 types of moment across the plasma membrane one is the active transport and the other is the passive transport. The active transport involves the help of ATP whereas the passive transport does not involve ATP to transport the materials.
Answer:
The division cycle of most eukaryotic cells is divided into four discrete phases: M, G1, S, and G2. M phase (mitosis) is usually followed by cytokinesis. S ...
Missing: 6 Mass (arbitrary units) 16 18
Explanation:
