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Tanzania [10]
3 years ago
7

What was chargaffs observation about the nitrogen bases in dna

Biology
1 answer:
zubka84 [21]3 years ago
5 0
DNA contains equal amounts of adenine/thymine and guanine/cytosine.
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Describe how a plasmid can be genetically modified to include a piece of foreign dna that alters the phenotype of bacterial cell
Ilia_Sergeevich [38]
Simple version:

First, the section with the desirable gene must be identified. Assuming that has already happened, the section of DNA must be excised from the original genome using restriction enzymes, which recognize certain DNA sequences and snip DNA at those sites. DNA ligase is used to "glue" these ends back together. The DNA is inserted into a plasmid (also with restriction enzymes), which would usually contain antibiotic-resistance genes (so they survive in an environment containing the antibiotic, which would also help show if the bacteria have been successfully transformed). 

Then comes the actual transformation process. The bacteria to be transformed are mixed with calcium chloride (which causes the bacteria to be more receptive to the plasmids) and then mixed with the plasmids. The bacterial cells are subjected to a heat shock (the solution is heated and rapidly cooled, e.g. by placing the mixture in a hot water bath and quickly transferred to ice) so they will take up the plasmid (since the temperature change makes the membrane more permeable). The bacteria are placed on a growth medium containing the antibiotic they're resistant to. Only those successfully transformed would survive.
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3 years ago
A nurse suspects that the iv line through which doxorubicin (adriamycin) is infusing has infiltrated. the nurse has discontinued
Ainat [17]
A nurse suspects that the IV line through which Doxorubicin (adriamycin) is infusing has infiltrated. The nurse has discontinued the IV site.
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6 0
3 years ago
Which explanation best explains the role of enzymes as catalysts in living systems? Enzymes accelerate chemical reactions withou
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First one is correct. ...remeber my teacher telling us enzymes never get used
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As boys grow, they become taller and gain body mass. during this time, muscle changes as well, first in _______________ and seco
Alla [95]
<span>The answer is mass and high-level force production. As boys grow, they become taller and gain body mass. During this time, muscle changes as well, first in mass and second in high level force production.</span>
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3 years ago
Which cellular process takes place in the ribosome that are bound to the endoplasmic reticulum
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Actually no cellular process takes place IN the ribosomes. 
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<span>And if it's the ribosomes associated with ER, then the proteins are going to be distributed to some other organelle in the cell or exported out of the cell.</span>
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3 years ago
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