1answer.
Ask question
Login Signup
Ask question
All categories
  • English
  • Mathematics
  • Social Studies
  • Business
  • History
  • Health
  • Geography
  • Biology
  • Physics
  • Chemistry
  • Computers and Technology
  • Arts
  • World Languages
  • Spanish
  • French
  • German
  • Advanced Placement (AP)
  • SAT
  • Medicine
  • Law
  • Engineering
SSSSS [86.1K]
4 years ago
12

The forward and reverse PCR primers used for the amplification of a VNTR locus on chromosome number 4 are 20 bases and 21 bases

long, respectively. The 3’ base of the forward primer is 53 bases away from the first repeat. The 3’ base of the reverse primer is 28 bases away from the first repeat in that direction. Each repeat unit is 60 bases in length. A person has, on one of their number 4 chromosomes, 6 repeats. How long (in bp) should its PCR product be?
Biology
1 answer:
zimovet [89]4 years ago
5 0

The repeats which occur together on a chromosome are referred to as VNTR. The length of each repeat is 60bp.

The first primer is 20bp and is 53 bp away from 1st repeat. Therefore, the total length of sequence amplified by the first primer till 1st repeat = 20+53 = 73bp. Beyond this point 6 repeats on 60 bp are present, hence, the length becomes = 73 + (60X6) = 433bp.

Now the reverse primer which is 21bp in length is located 28bp from the repeat on its side. Reverse primer comes from the opposite direction, so it must be present after the 6 repeats.

The total length of the amplified region = 433 + 28 + 21 = 482 bp.

You might be interested in
Please I need as much help as possible as quick as possible!How would you compare a plant cell to earth?
timama [110]
You could say the core of the earth is equal to the nucleus of the cell and the outer layer of the earth is like the cell wall
7 0
3 years ago
If the mitochondria and chloroplasts in eukaryotic cells resulted from endosymbiosis between various prokaryotes, what features
const2013 [10]

Answer: A plasma, membrane, DNA and ribosomes

Explanation:

3 0
1 year ago
Why do scientists study fossils?
Tasya [4]
Hi,

By studying fossils, scientists can learn how life has changed over time, how earth's surface has changed and what past environments were like. Fossils form when living things die and are buried by sediment; they are usually found in Sedimentary rock. Only the hard parts of organisms generally leave fossils because the soft parts generally decay too quickly to fossilize. In case you didn't know, there are two types of fossils; rock fossils and preserved fossils.

Option c) is the correct option above.

<span>c) Fossils give information about the time period in which organisms lived in the past. 



Faith xoxo</span>
6 0
3 years ago
What is a characteristic that all songbirds share in Florida?
Sladkaya [172]
I believe would think D.
3 0
3 years ago
Read 2 more answers
What forms between a positive and negative ion
xxTIMURxx [149]

Answer:

An ionic bond.

Explanation:

A strong electrostatic force of attraction will develop between the oppositely charged ions. For example, sodium chloride (salt) is essentially made of a positively charged sodium ion and a negatively charged chloride ion.

3 0
3 years ago
Other questions:
  • As a result of nondisjunction, a female gamete may contain
    14·1 answer
  • 1. What is a moments of a force?
    9·1 answer
  • Primary succession occurs in an area where an ecosystem once existed true or false
    10·2 answers
  • What is the function of a cell wall?
    12·1 answer
  • Alex sold 330 candy bars to raise money for his community basketball league. Milk chocolate candy bars sold for $2 each, and whi
    9·2 answers
  • Some types of bacteria are able to perform photosynthesis. these bacteria must therefore contain.
    6·2 answers
  • What two substances are needed for cellular respiration
    14·1 answer
  • Please help me with answer
    8·2 answers
  • Ventricles _____.
    6·2 answers
  • Crude oil is turned into usable forms of energy in a ______.
    12·1 answer
Add answer
Login
Not registered? Fast signup
Signup
Login Signup
Ask question!