1answer.
Ask question
Login Signup
Ask question
All categories
  • English
  • Mathematics
  • Social Studies
  • Business
  • History
  • Health
  • Geography
  • Biology
  • Physics
  • Chemistry
  • Computers and Technology
  • Arts
  • World Languages
  • Spanish
  • French
  • German
  • Advanced Placement (AP)
  • SAT
  • Medicine
  • Law
  • Engineering
SSSSS [86.1K]
3 years ago
12

The forward and reverse PCR primers used for the amplification of a VNTR locus on chromosome number 4 are 20 bases and 21 bases

long, respectively. The 3’ base of the forward primer is 53 bases away from the first repeat. The 3’ base of the reverse primer is 28 bases away from the first repeat in that direction. Each repeat unit is 60 bases in length. A person has, on one of their number 4 chromosomes, 6 repeats. How long (in bp) should its PCR product be?
Biology
1 answer:
zimovet [89]3 years ago
5 0

The repeats which occur together on a chromosome are referred to as VNTR. The length of each repeat is 60bp.

The first primer is 20bp and is 53 bp away from 1st repeat. Therefore, the total length of sequence amplified by the first primer till 1st repeat = 20+53 = 73bp. Beyond this point 6 repeats on 60 bp are present, hence, the length becomes = 73 + (60X6) = 433bp.

Now the reverse primer which is 21bp in length is located 28bp from the repeat on its side. Reverse primer comes from the opposite direction, so it must be present after the 6 repeats.

The total length of the amplified region = 433 + 28 + 21 = 482 bp.

You might be interested in
Which muscle group works the hardest isometrically when performing a wrist curl in pronated position
klemol [59]
I am pretty sure that would be <span>wrist flexors</span>
7 0
3 years ago
I need help please can anyone help?
GenaCL600 [577]
Cell wall.

Made of cellulose and acts as a stronger cell membrane in a plant cell
4 0
2 years ago
Read 2 more answers
Choose the incorrect statement concerning centrioles. A) oriented at right angles to each other B) found in both prokaryotes and
Korolek [52]
The answer is B). Because Centrioles play role in Mitosis. Prokaryotes don't divide by Mitosis. They divide by binary fission.
8 0
3 years ago
Origin and Expansion of the Universe Lab Report
Mandarinka [93]

Answer:

this is for number 6

Explanation:As the universe continues to expand, the gravitational force between the stars will decrease the farther apart they become.

8 0
3 years ago
How do sedimentary rocks form on Earth’s surface?
erik [133]

Answer:

I am 95% sure its b, if not then it is c

5 0
3 years ago
Other questions:
  • Which major tissue category includes tissues that function in secretion, absorption, and filtration?
    11·1 answer
  • What is the average density of the Earth's inner core?
    5·1 answer
  • All organisms need ______ to survive.​
    11·2 answers
  • How do fronts change weather?
    8·2 answers
  • 5. Which of the following will most likely disrupt the stability of an ecosystem?
    5·1 answer
  • Develop a scenario with a Punnett Square and discuss how the offspring outcomes change if the gene
    6·1 answer
  • How does natural selection provide a mechanism for evolution to occur?​
    12·1 answer
  • What are the functions of the pioneer community look at the pic
    15·1 answer
  • Property of water that makes transpiration to occur
    5·2 answers
  • A(n) is a protein and/or sugar molecule on the surface of a cell that allows the immune system to identify whether or not that c
    12·1 answer
Add answer
Login
Not registered? Fast signup
Signup
Login Signup
Ask question!