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Lady bird [3.3K]
3 years ago
9

Please help me on these

Biology
2 answers:
Nina [5.8K]3 years ago
4 0
Wind power and heat from sun.
Otrada [13]3 years ago
3 0
Wind Power and the heat from the sun
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Several cell processes that take place during the cell cycle are listed below. A student notices that the processes are listed i
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Design an experiment to answer the question, what is the effect of caffeine on the rate of mitosis in frog eggs? Label: hypothes
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Answer:

Explanation:

Null Hypothesis: Their will be no effect of the caffeine on the mitosis.

Hypothesis: Caffeine will inhibit the SHSY5Y cell division.

Dependent variable: Various concentration of the caffein compound.

Independent Variable: temperatures of the experimental condition. If their will be any effect of the temperature in mitosis then it will also affect the mitosis of control cells.

Experimental design.

Culture SHSY5Y neuronal cell line in the T-75 culture flask in CO2 incubator at 37 degree celsius. Next day, add cells from T-75 flask in the 24 well plate and allow cells to adhere to the well by incubating plate in CO2 incubator at 37 degree celsius. Add the compound caffeine at different concentration (say 1 microgram, 2 microgram, 3 microgram, 4 microgram) and incubate the plate for 24 hour. Determine the mitotic cells by following assay.

Assay to determine mitotic cell.

The reagent succinimidyl ester of carboxyfluorescein diacetate (CFSE) binds to cytoplasmic proteins. it is fluroscent reagent. And when cell divide due to mitosis, cytoplasm will also divide and hence, the protein which stained with the CFSE reagent will also goes in the daughter cell. Then, the fluorescence of the stained cells can be read on the plate reader.

Prepare the graph of the fluorescence values (Y-axis) verses different concentrations of the caffein (X-axis) and determine the slop of curve to know the rate of mitosis.<u> </u>

<u>Following test and control samples will be used for the assay.</u>

1. SHSY5Y cell line with added caffeine. It is test sample.

2. SHSY5Y cell line added with buffer. This important to know behaviour of cells under experimental conditions.

3. SHSY5Y cell line with solvent used to dissolve compound caffeine. It is important to know the toxicity or any effect of solvent on the mitosis.

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Consider the population of four juvenile condors their weights in pounds are
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Their weights in pounds are 5, 7, 10, 11.
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Which region of the human alimentary tract has both the largest population of bacteria and the greatest species diversity
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The region of the human alimentary tract that has both the largest population of bacteria and the greatest species diversity is the the colon.

<h3>The normal flora of the alimentary tract</h3>

Normal flora are those microorganisms that resides in a living organism without causing it harm or diseases.

These normal flora become established in different parts of the body from birth such that the normal flora found in the mouth is not the same as the one found in the colon of the alimentary tract.

From research works, the large intestine (colon) is known to contain 100 billion per milliliter of fluid culture.

The massive numbers of bacteria in the large intestine (colon) creates a great special variation in the flora. The streptococci and lactobacilli found in the colon often adhere to cells by means of capsules surrounding the bacteria.

Therefore, the region of the human alimentary tract that has both the largest population of bacteria and the greatest species diversity is the the colon.

Learn more about normal flora here:

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