Answer:
To produce a terminator nucleotide in Sanger DNA sequencing, a(n)<u> oxygen </u>atom is removed from the hydroxyl group on the deoxyribose sugar where it bonds with the adjacent phosphate group
Explanation:
DNA consists of a double helix made up of nucleotides.These nucleotides are arranged in such a way that each nucleotide has a phosphate group , a sugar and a nitrogenous base.
Sanger sequencing is a method of DNA sequencing which is actually based on the selective incorporation of the chain-terminating dideoxynucleotides by DNA polymerase during the DNA replication
The Sanger sequencing has following steps :
1) denature dsDNA using heat.
2) Make multiple copies of a segment.
3) Attach a primer.
4) Add to four polymerase solutions.
5) Grow complementary chains until termination dye.
6) Denature the grown chains.
7) Electrophorese the four solutions.
