Answer: A. Mesopelagic zone
Explanation: This zone is referred to as the "middle zone" within oceanic waters. Not yet the deep zone, however past the top layer aka upper ocean.
Hope this helps ^^
1: I believe is B.
2: I believe is True
3: I believe is D.
4: I believe is D.
5: I'm not completely sure about this one, because it depends on the substance. Rocks molecules don't move because the object is solid, no matter how cold or hot the rock is. But if I were to guess, I would say True.
6: I believe is A.
7: I believe is B.
8: I believe is False.
9: I believe is False.
10: I believe is C.
11: I believe is False.
12: I believe is C.
13: I believe is A.
14: I believe is A.
I really hope I got all of these correct. Hope this helped!
-TTL
Nicked circle is the reason.
Explanation:
During recombinant DNA technology the gene of interest is ligated to plasmid DNA by excision with molecular scissors or endonuclease enzyme and ligated with enzyme ligase.
Enzyme ligase catalysed by ATP seals the nicks by making phosphodiester bonds in the ds or ss DNA and release of ADP.
The ligation in the plasmid did not occur properly hence the replication is interrupted hence no new plasmids with gene of interest replicated in the ligation carried out.
Answer:
ur answer A is correct i think-
Explanation: