Answer:
The correct option is 3. "At pH 6.5 the enyzme is 50% active" 
Explanation:
For the titratable group to be protonated and cause the enzyme to be in the active state, it needs to have gained a hydrogen cation (H+). In order for that to happen, there must be enough hydrogen cations in the environment of the enzyme, and hence, an acidic pH is required in this case.
 
        
             
        
        
        
The new leaf will contain 14 chromosomes, because during mitosis, a cell makes an exact copy of itself. Therefore, the number of the chromosomes in that cell stay the same. 
        
                    
             
        
        
        
A. the kelvin temperature scale