The "scissors" of the molecular biology are: DNA Restriction enzymes.
A restriction enzyme is a protein capable of cleaving a DNA fragment at a characteristic nucleotide sequence called a restriction site. Each restriction enzyme thus recognizes a specific site. Several hundred restriction enzymes are currently known.
Naturally present in a large number of species of bacteria, these enzymes have become important tools in genetic engineering.
The "glue" of the molecular biology are: DNA ligase
In molecular biology, DNA ligases are ligase-class enzymes that catalyze the formation of a phosphodiester bond between two segments of DNA. DNA ligases are involved in several essential cellular processes of DNA metabolism: in DNA replication, suture of Okazaki fragments, and in DNA repair and homologous recombination.
The use of these tools in molecular biology: Cloning
Molecular cloning is one of the bases of genetic engineering. It consists of inserting a DNA fragment (called insert) in an appropriate vector such as a plasmid for example. The new plasmid thus created will then be introduced into a host cell, generally the Escherichia coli bacterium. This will then be selected and multiplied to obtain a large amount of the plasmid of interest. Cloning a gene involves inserting it into a plasmid. A clone will be the bacterial transformant that contains this particular plasmid. In this case we speak of clone because all the individuals of the bacterial colony are genetically identical. Molecular cloning is thus different from reproductive cloning (creating an individual genetically identical to another but of a different age) or therapeutic cloning (making tissues from stem cells to perform transplants compatible with the recipient).
Molecular cloning requires restriction enzymes capable of cleaving the DNA, and DNA ligase capable of re-gluing the DNA fragments. Ligase was isolated for the first time from T4 bacteriophage. This enzyme is involved in the repair and replication of DNA. It can bind DNA fragments with compatible sticky ends. At higher concentration, this enzyme is also able to bind two ends of DNA as shown here. T4 DNA ligase works using ATP and Mg ++. It has an activity optimum of 16 ° C, but remains active at room temperature.
Answer:
Glucose is like the tank of the delivery truck because it transports a relatively large amount of energy to cells. ATP is like the gas tank of the car because it contains a small amount of energy that is used to carry out the work of individual cells.
Individual and public health are affected by environmental and genetic factors is a statement which reflects reality. Firstly, susceptibility to certain diseases usually or often runs in a family so that if there is say, diabetes in the father and grandmother for example, the son or daughter has more chances of getting it so this is a genetic susceptibility.. Also, some families are more prone to cancer so that given the right circumstances, cancer can develop. So it is usually a combination of both genetic and environmental causes that results in a disease. An example of public health could be when smallpox was deliberately introduced to the First Nations people in British Columbia, Canada back in the 1800's to decimate them and remove their resistance to the gold mining hordes. Since the First Nations had no anti-bodies to counteract such a disease unlike the white men who introduced it to them, then they easily fell prey to it and died within days of contracting it.
