Multiple approaches are commonly used to study the mechanism of action of ... The study of reaction rates and how they change in response to changes in ... Substrate Concentration Affects the Rate of Enzyme-Catalyzed Reactions ... its substrate molecule to form the ES complex as a necessary step in enzyme catalysis.
The intracellular is where enzymes need to perform optimally or near optimum.
Explanation:
The Km is the concentration of molecules where an enzyme performs at half of its maximum velocity (Vmax). Therefore, when molecules are near Km the enzyme is able to hydrolyze molecules nearer its Vmax.
