Hershey and Chase used radioactive phosphorus and sulfur to label T2 bacteriophage. Then they infected E.coli with these bacteriophage labeled with radioactive phosphorus and sulfur.
After infection, they centrifuge the cell and found that radioactive phosphorus is present in the bottom and radioactive sulfur in supernatant . As sulfur is mostly the part of protein not DNA and is found in supernatant and phosphorus is mostly present in DNA not protein which was found in cell debris, therefore, it was proved that DNA is the genetic material.
Hershey and chase in 1952 performed an experiment to prove that DNA is genetic material by the bacteriophages is inserted into the bacteria as soon as it lives with the host.
In this experiment, the bacteriophage forced to bacterial cells after adsorption by use as a very high-speed blender. After the adsorption to the bacteria, lack of radioactive phosphorus(32) labeled DNA found in solution which moves to the bacterial cell from the phage. The presence of the radioactive sulfur in the solution states that the protein coat stays outside of the cell.
Thus, the correct answer is - phosphorus and sulfur.
