This would cause a cut at the wrong recognition site. As a result, a defective transformation would occur.
Explanation:
It is necessary to understand that restriction enzymes are conditioned to cut specific parts of DNA, which they recognize through a sequence of specific bases. The recognition of genes by this enzyme is also something of extreme importance so that no harm is done to the DNA that leads to the formation of defective structures in the body.
Based on this, we can say that if a cut was made in the medusa glo gene and the plasmid puc18 with the restriction enzyme ecor1, we would cause a cut at the wrong recognition site. As a result, a defective transformation would occur.
On the off chance that there is an EcoR1 site in either the center of the Glo quality, or amidst the selectable marker site in the plasmid, it would likely debilitate either Glo, or the plasmid.I hope this will help.
