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cupoosta [38]
3 years ago
10

What would have happened if we had cut both the jellyfish glo gene and puc18 plasmid with the ecor1 restriction enzyme?

Biology
2 answers:
pychu [463]3 years ago
6 0

Answer:

This would cause a cut at the wrong recognition site. As a result, a defective transformation would occur.

Explanation:

It is necessary to understand that restriction enzymes are conditioned to cut specific parts of DNA, which they recognize through a sequence of specific bases. The recognition of genes by this enzyme is also something of extreme importance so that no harm is done to the DNA that leads to the formation of defective structures in the body.

Based on this, we can say that if a cut was made in the medusa glo gene and the plasmid puc18 with the restriction enzyme ecor1, we would cause a cut at the wrong recognition site. As a result, a defective transformation would occur.

nekit [7.7K]3 years ago
4 0
On the off chance that there is an EcoR1 site in either the center of the Glo quality, or amidst the selectable marker site in the plasmid, it would likely debilitate either Glo, or the plasmid.I hope this will help. 
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In dogs, wire hair is due to a dominant gene (W), smooth hair id due to its recessive allele.a. If a homozygous wire-haired dog
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Answer:

a) All the offspring produced would be a heterozygous in the first generation (F1 generation) with genotype: Ww, Ww, Ww, and Ww. Since wire hair is dominant, all would be wired haired.

b) In the F2 (second generation), we breed the heterozygous offspring from the F1 generation together (Ww x Ww). This will produce 3:1 ratio of dominant:recessive phenotypes having 3/4 of the offspring wire-haired (1/4 WW homozygotes and 1/2 Ww heterozygotes) and 1/4 will be smooth-haired (ww). Also the genotype would have the ratio 1:2:1 (i.e 1 homozygote WW, 2 heterozygote Ww and 1 smooth hair)

c) the chances of producing a smooth-haired pup is 1/4, and the chances of producing a wire-haired pup are 3/4.

d) Therefore, 1/2 of the offspring will have the genotype Ww and be wire-haired, and 1/2 of the offspring will be ww and be smooth-haired.  Also the phenotype ratio is 1:1 (1/2 is heterozygote wired hair and 1/2 is smoth haired)

Explanation:

Since the wire hair is the dominant gene (W) and smooth hair (w) is the recessive allele

a)  If a homozygous wire-haired dog is mated with a smooth-haired dog, All the offspring produced would be a heterozygous in the first generation (F1 generation) with genotype: Ww, Ww, Ww, and Ww. Since wire hair is dominant, all would be wired haired.

b) In the F2 (second generation), we breed the heterozygous offspring from the F1 generation together (Ww x Ww). This will produce 3:1 ratio of dominant:recessive phenotypes having 3/4 of the offspring wire-haired (1/4 WW homozygotes and 1/2 Ww heterozygotes) and 1/4 will be smooth-haired (ww). Also the genotype would have the ratio 1:2:1 (i.e 1 homozygote WW, 2 heterozygote Ww and 1 smooth hair)

c) If two wire-haired dogs produce a smooth-haired pup, that means that both parents must be heterozygotes (Ww) having a pair of dominant W allele and recessive w allele to pass on to the offspring. Therefore, if these two dogs were to mate again (Ww x Ww), the chances of producing a smooth-haired pup is 1/4, and the chances of producing a wire-haired pup are 3/4.

d) If the mother of the wire-haired male was smooth-haired, that means that the recessive allele w had been passed on to the male making the male a   heterozygote (Ww). When this male mates with a smooth-haired female (ww), the cross is Ww x ww. Therefore, 1/2 of the offspring will have the genotype Ww and be wire-haired, and 1/2 of the offspring will be ww and be smooth-haired.  Also the phenotype ratio is 1:1 (1/2 is heterozygote wired hair and 1/2 is smoth haired)

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Hints:

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