In situ hybridization is a powerful tool used in gene expression studies because it provides the investigator with information p
ertaining toA)the cellular and tissue-specific localization of the mRNA encoded by a particular gene.B)the activity of the protein translated from a particular mRNA.C)the size of the mRNA transcript.D)all of the above
A)the cellular and tissue specific localization of the mRNA encoded by a particular gene
In situ hybridization is a technique used to locate and detect nucleic acid sequences(DNA or RNA) with respect to their protein product within nuclei
The principle behind in situ hybridization is that specific annealing(heat treatment process) of labelled probe to complementary sequences of target DNA or mRNA in a fixed specimen is done
Probe is mainly used to find the complementary sequence of the nucleic acids(DNA or RNA) or helps in localization of particular clone
The detection and visualization of the hybrids can be done by using cytological methods
