When cloning by restriction digest and ligation, you use restriction enzymes to cut open a plasmid (backbone) and insert a linear fragment of DNA (insert) that has been cut by compatible restriction enzymes. An enzyme, DNA ligase, then covalently binds the plasmid to the new fragment thereby generating a complete, circular plasmid that can be easily maintained in a variety of biological systems. Read on for an in-depth breakdown of how to do perform restriction digests.
Answer:
Your answer is 2.
Explanation:
To calculate the mechanical advantage of a pulley you simply have to count the number of rope sections that support whatever object you are lifting (not counting the rope that is attached to the effort). For example, in a one pulley system the MA is 1. In a two pulley system the MA is 2.
High concentration and passive transport
PLATO USERS
Intertidal Zone 10 m (33 ft)
Sublittoral Zone 200 m (660 ft)
Hadal Zone 10,911 m (35,797 ft)
Bathyal Zone 6,000 m (19,686 ft)
[ a ] the sublittoral zone or the shallowest bathyal zone
[ b ] the intertidal zone or the deepest hadal zone
[ c ] the oceanic zone or the deepest intertidal zone
[ d ] <em><u>"The Intertidal Zone Or The Shallowest Sublittoral Zone."</u></em>
Answer:
co2
Explanation:
Hope this helps, have a nice day!
