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Nonamiya [84]
3 years ago
13

During the spread plate method of isolation, a few drops of the initial culture is transferred to tube

Biology
2 answers:
sesenic [268]3 years ago
8 0
The answer is a loop of the tube culture
notsponge [240]3 years ago
8 0

Answer:

Tube A will produce a higher number of CFUs on the plate

Explanation:

This procedure is called dilution: when a loop of the previous (and more concentrated culture; <em>tube A</em>) is diluited in new culture medium, few microorganisms disperse in the new liquid. This results in a less concentrated culture (<em>tube B</em>).

When drops of this diluited culture (tube B) are spread in a plate, microorganisms can grow far from each other and iniciate an isolated colony. A lower number of colonies will grow from this tube.

Results of the spread method of isolation: with enough dilution, each colony is generated from a single microorganism. Then, all the microorganisms in a colony have grown from a single cell and they have the same genetic material (they are <em>genetically identical</em>)

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