1answer.
Ask question
Login Signup
Ask question
All categories
  • English
  • Mathematics
  • Social Studies
  • Business
  • History
  • Health
  • Geography
  • Biology
  • Physics
  • Chemistry
  • Computers and Technology
  • Arts
  • World Languages
  • Spanish
  • French
  • German
  • Advanced Placement (AP)
  • SAT
  • Medicine
  • Law
  • Engineering
Mkey [24]
4 years ago
5

A DNA sequence of interest was digested and ligated into the middle of the AmpR gene region of pBR322 vector. The vector also co

ntains a TetR gene which is not interrupted. After introducing the recombinat DNA into bacterial cells, the resulting transformants were tested on a variety of media. Which of the following results would be expected?
A) no growth on either ampicillin or tetracycline containing mediaB) growth on ampicillin but not tetracycline containing mediaC) growth on tetracycline but not ampicillin containing mediaD) growth only on media containing both antibioticsE) growth on both ampicillin containing media and tetracycline containing media
Biology
1 answer:
svp [43]4 years ago
7 0

Answer:

B

Explanation:

We know that the AmpR gene region has been interrupted. So this means the AmpR gene will not function in the recombinant DNA.

But the question tells us that the TetR gene is not interrupted, so the TetR gene will still function.

AmpR represses the protein that confers resistance to ampicillin, and AmpR is no longer functional, <u>so the new bacterial cells will be resistant to ampicillin.</u>

TetR represses the protein that causes resistance to tetracyline, and TetR is still functional, <u>so the new bacterial cells will not be resistant to tetracycline. </u>

This means B, there will be <u>growth on ampicillin but not tetracycline containing media.</u>

You might be interested in
Umami is one of the taste types that humans can recognize. Which selections below describes the taste of umami?
alexandr402 [8]
Umami is found in foods such as seaweed, miso, mushrooms, and parmesean cheese... It generally tastes “savory,” but I wouldn’t say that it tastes “meaty.” A or B is likely your best bet.
3 0
3 years ago
Are there organelles that only exist in animal cells? If so, state it and it's<br>function.​
Igoryamba
Animal cells each have a centrosome and lysosomes, whereas plant cells do not. Plant cells have a cell wall, chloroplasts and other specialized plastids, and a large central vacuole, whereas animal cells do not.
3 0
3 years ago
23. When washing tableware in a three compartment sink, the water temperature should be at least ) 90F (32 degrees) 100 degrees
nadya68 [22]

Answer:

The water temperature should be at least 110^{0}F (43^{0} C).

Explanation:

Temperature is a measure of the degree of hotness or coldness of a substance. It is measured either Kelvin, in degree Fanrenheit or Celsius. These scales can be converted from one to another.

To ensure appropriate or proper cleaning of table wares, hot water at required temperature is adviseable for use. This is because most germs can not survive under hot condition, thus they die.

Washing with water of at least 110^{0}F (43^{0} C) temperature in a three compartment sink helps to kill germs on the table ware.

5 0
3 years ago
A substance that speeds up the rate of a chemical reaction is called
aksik [14]
A substance that speeds up the rate of a chemical reaction is called a Catalyst.
7 0
4 years ago
Read 2 more answers
Which factors are involved in earthquake formation? Check all that apply. plates moving rocks breaking stress that decreases fau
AfilCa [17]

The correct answers are:

- plates moving;

- breaking rocks;

- movement bellow the surface;

The earthquakes are a direct result of the plate tectonics. In order for an earthquake to appear, there should be a geologic activity caused by the movement of the tectonic plates. The movement of the tectonic plates causes a lot of pressure on the rocks. The rocks eventually break at certain places under the pressure. A they break, there's movement bellow the surface, or rather inside the crust, also referred to as adjustment. That movement and the release of power from it, causes lot of strong vibrations. The vibrations spread out and start shaking the ground in the surrounding area, which is actually the earthquake itself.

5 0
3 years ago
Read 2 more answers
Other questions:
  • In a certain area, mosquitoes that can resist the effects of pesticides (insect-killing chemicals) now exist in greater numbers
    11·1 answer
  • The metabolic disturbances of diabetes are thought to predispose peripheral nerves to injury by:
    9·1 answer
  • Which process in the body is primarily controlled by testosterone? A.Implantaion of embryo B.production of ovum C. formation of
    7·1 answer
  • The endocrine system can influence behavior of an animal as early as embryonic development. This is considered an _____ effect.
    12·1 answer
  • what is the advantage of timing over a shorter period of time especially when you have just finished exercising
    5·1 answer
  • What information did you include in your
    5·1 answer
  • Arrange these health risks in decreasing order of the frequency at which they cause death among adolescents.
    11·1 answer
  • The complex structures of DNA and protein found in the cell nucleus are mitochondria. nucleoplasm. histones. nucleases. chromoso
    11·1 answer
  • Answer the following in several complete sentences:
    10·1 answer
  • While doing field research, two scientists discover a new species of plant. They take the plant back to their lab to watch how i
    13·1 answer
Add answer
Login
Not registered? Fast signup
Signup
Login Signup
Ask question!