Question

What are the main processes of protein structure analysis by frozen electron microscopy?

Answer 1

Answer:

The analysis of protein structure by frozen electron microscopy can be divided into three processes：

1. Sample preparation: the biomacromolecule sample is frozen rapidly in liquid nitrogen, and the water in the macromolecule exists in the form of glass.

2. Data collection: irradiate the sample and the ice near the sample with low energy electron beam, and record the scattered signal with detector.

3. Three dimensional reconstruction to build protein model: three-dimensional structure of protein is built by two-dimensional projection from different angles.

Explanation:

https://www.creative-biostructure.com/Data-Processing-and-Interpretation-584.htm