Definition
A technique which is used to separate, DNA, RNA or protein pieces from each other under the influence of electric field on the basis of their molecular size is known as gel electrophoresis.
Explanation
This method is very reliable for separation of large size molecule (over 1 million Da). Materials which are required for gel electrophoresis include:
1. TAE stock buffer
2. 1% agrose gel
3. Nucleic acid loading dye
4. Ethidium bromide
Procedure:
First prepare a stock solution of TAE buffer by adding appropriate amount of TAE in distilled water. then prepare 1% agrose by adding 1X TAE, some amount of agrose in water and heating it in microwve oven to mix them will. then pour agrose gel on tray and fix comb in it and keep it untill agrose dry. Then remove comb and pour some quantity of nucleic acid along with loading dye and ethidium bromide in each well. EtBr is used for staining nucleic acid.
When sample is poured in all well also pour reference marker in one well for comparison. now connect it with voltage for 30-35 min. After this take gel and see it under UV. a large number of nucleic acid pieces will be seen on gel under UV. those pieces which have small molecular weight will cover more distance compared to those having larger molecular weight.
The general function of the endoplasmic reticulum is to produce protiens for the cell to function. The rough ER in particular has many ribosomes on it's surface, while the smooth ER does not. These ribosomes give the rough ER it's distinct appearence under a microscope, and assist in making protiens.
Answer:
Mitochondrial DNA is circular, so it doesn't shorten when it replicates unlike the rest.
Answer:
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Earth can be divided into three MAIN layers: the core, the mantle and the crust. Each of these layers can be further divided into two parts: the inner and outer core, the upper and lower mantle and the continental and oceanic crust. ... The inner core is solid, while the outer core is liquid.
