Answer:
The effect of amanitin on the maximum elongation rate for the wild-type and modified RNA polymerases is that it binds to the RNA polymerases, and reduces the process of translocation which is essential for RNA synthesis that is required for RNA polymerases elongation.
Explanation:
Amanitin is a peptide that is cyclic in nature. It is repelled by water thereby making it an hydrophobic peptide.
Amanitin is a toxic peptide that is found in Amanita ( a type of mushroom).
Alpha Amanitin in particular is the one that affects the elongation rate of RNA Polymerases in the body.
When Alpha Amanitin gets into the body system, it travels straight to the liver and due to its very strong affinity for RNA polymerases, it immediately attaches itself to them.
After the attachment, Alpha Amanitin, is disturbs the bridge helix found in RNA polymerase, preventing the hindering and slowing down the proces of translocation from happening.
Once translocation is hindered, RNA is no longer synthesized. Hence, the elongation of RNA polymerases is hindered and this results in severe illness in the body such as liver failure, cytolysis of the liver
LIt will increase steadily over time
The process of osmosis explains what happened to the cells.
Osmosis is where water molecules will flow from a region of higher water potential to a region of lower water potential, through a partially permeable membrane. Red blood cell is a human cell and have a partially permeable membrane. At the same time, pure water has the highest water potential among all substances, and of course higher than the cytoplasm of the red blood cell, therefore, water molecules will flow from the water to the red blood cell.
So much water has flown to the red blood cells that the cell membrane cannot hold all and eventually the cell bursts. Therefore, the process of osmosis explains this result.
These microbial infections that are acquired by hospital patients as they are being used as the compromised host are the nosocomial infections. These type of infections are primarily caused by bacterial pathogens entering the patient's body and mainly causes urinary tract infection, pneumonia, and other illnesses.