Answer:
DNA restriction enzymes cut the DNA molecule, while DNA ligases join the resulting DNA fragments
Explanation:
Transformation is a naturally occurring process by which bacteria incorporate exogenous genetic material from their surrounding environment. This process (transformation) is used for DNA cloning via plasmid vectors. In DNA cloning, transformation occurs after restriction enzymes cut the DNA at specific sequences named palindromic sequences (i.e, sequences that can be read the same in opposite direction). Restriction enzymes can generate sticky-ends, where enzymes make staggered cuts in the two strands (e.g., <em>BamH</em>), or blunt ends, where the resulting strands are of the same length (e.g., <em>HaeIII</em>). In general, sticky-end enzymes are more useful because they generate a 3' overhang in one molecule and a complementary 5' overhang in the other, increasing the yield and specificity of ligation. During ligation, a DNA ligase is used to join both DNA strands by forming phosphodiester bonds in the plasmid. Following transformation, bacteria can be selected on antibiotic plates.
It is important to study the cell in order to learn about DNA is that there many different processes that help to keep a cell running. In order to know DNA, you need to familiarize yourself with mRNA, tRNA, and rRNA to learn the process of transcription and translation- two very important processes in DNA.
OK so for this one it is quite simple when we clear forests we basically take away an insects habitat therefore by clearing a forest for our personal use it will have the greatest impact on the insects. So the best choice to go with will be option B.
Disease and salinity is the answer you are looking for
