Question

In one publication, the author implanted biomaterials subcutaneously in rats to test the biocompatibility of materials. At a few time points, the animals were sacrificed and the author performed H&E staining on the tissue samples. The thickness of fibrous capsule layer was then measured by calculating the average width between the two dash lines in a few H&E images of the same sample but different sections. What is the major problem of this measurement? (10 points)

Answer 1

Answer:

That the material can be mixed with the original tissue

Explanation:

The biomaterial has the idea to be mixed with the original tissue, due is going to grow (if it is based in cells) or is going to attach to the biological tissue, so the H&E method can be confused. The hematoxylin is going to stain the nuclei of the cells meanwhile the eosin is going to stain the extracellular matrix and cytoplasm, so, if the biomaterial is mixed in the original tissue, can be confused the exactly boundary among the biomaterial and the biological tissue.

Hope this info is useful.