answer: it increases from left to right
explanation:
the electronegativity of atoms increases from left to right across a period in the periodic table; it also decreases as you move from top to bottom down a group in the periodic table
The use of a thermophilic DNA polymerase such as Taq polymerase prevents the denaturation of the enzyme during the heating that is important to separate the newly synthesized strand. So using this enzyme simplifies the PCR technique and increases its efficiency.
Taq DNA polymerase is highly efficient, when it reaches the optimum temperature, it becomes fully functional. It also has a half-life of over two hours (at 92°C), high amplification capacity, and it can add upto 150 nucleotides per second. It is "special" enzyme because it comes from the bacterium Thermus aquaticus, which lives in hot springs. It is therefore thermostable even at high temperatures, while other polymerases (e.g. E. coli) are not.
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Answer:
The purine ring is built onto ribose-5-phosphate of PRPP for its de-novo nucleotide biosynthesis, while the ring structure of the pyrimidine bases are synthesized separately and then coupled to ribose-5-phosphate via the C-N glycosidic bond.
Explanation:
In the de novo synthesis of nucleotides, their metabolic precursors such as aminoacids, ribose-5-phosphate, CO₂ and NH₃ are used as starting materials.
In purine nucleotide synthesis, the ring structure is built up on ribose-5-phosphate of PRPP by addition of one or a few atoms one at a time starting with the amino group donated by glutamine until the first intermediate inosinate is synthesized.
In pyrimidine ring synthesis, orotate is first synthesized from carbamoyl phosphate and aspartate, and then is attached to ribose-5-phosphate of PRPP, before it is then converted to the common pyrimidine nucleotides starting from uridylate.
