Question

Which of the following describes the correct stepwise assembly (in vitro) of the eukaryotic RNA polymerase II preinitiation complex? TFIIA → TF11B → TFIID/TBP → TFIIE → TFIIH ( TFIIF/RNA Pol II TFIIA → TF11B → TFIID/TBP → TFIIF/RNA Pol II → TFIIE → TFIIH TFIID/TBP → TFIIA → TF11B → TFIIE → TFIIH → TFIIF/RNA Pol II TFIID/TBP → TFIIA → TF11B → TFIIF/RNA Pol II → TFIIE → TFIIH

Answer 1

Answer: (TFIID) TBP-> TFIIB IIA--> TFIIF RNA POL II

Explanation:

Remember that in eukaryotic organisms, transcription and processing are coupled processes. There are 3 different types of Rna pol. In the case of RNA pol II transcribes genes that encode proteins (mRNA synthesis). Transcription factors are involved, for example: TFIID, TBPs, TAF (recognizes TATA promoter center, regulatory functions), TFIIA (stabilizes TBP union, antirepressant function), TFIIB (RNA Pol II starting point selection).