Question

In a fluorescence recovery after photobleaching (FRAP) experiment, a fluorescently tagged membrane protein, A, shows very little recovery of fluorescence ten minutes after photobleaching, while membrane protein B shows a rapid increase in fluorescence after bleaching, recovering nearly 80% of its original fluorescent signal by ten minutes. Based on this information, which of the following statements can be made?
A. Proteins A and B interact with each other in a complex
B. Protein A is easily denatured,
C. Protein Bis diffusing in a more fluid membrane.
D. Protein Ahas faster mobility in the membrane.

Answer 1

Answer:

The correct answer is option C.  Protein Bis diffusing in a more fluid membrane.

Explanation:

Fluorescence recovery after photobleaching or FRAP is a technique used to examine the portability of fluorescent particles with the help of fluorescence microscopy that have been utilized since the mid-70s to evaluate the dispersion or movements of molecules in the cell membranes of the cell.

Fluorescence Recovery After Photobleaching (FRAP) is a strategy for deciding the energy of dispersion in living cells (typically) utilizing fluorescence microscopy.

In the given experiment:

The diffusion coefficient of GFP vary, based on the localization of the protein

Protein B is freely diffusing, due to which recovery is very high compared to protein A.

Protein which is less diffusing shows a slower and less rate of recovery.